What is the lowest reasonable melting temperature for PCR primers? - primer annealing temperature real time lab on web
... Taq polymerase used. Temp default extension of 72 degrees. I had a brain fart and ordered primers with a melting point of 40 degrees (if you're by the rule of 2 / 4) and I'm not sure if this is the annealing 30something and see what happens, or new primers, which longer have to.
Tuesday, February 2, 2010
Primer Annealing Temperature Real Time Lab On Web What Is The Lowest Reasonable Melting Temperature For PCR Primers?
1 comment:
Arent most expensive primers. We ask the RTD as $ 10 per 20 bp primers or less. This realignment should not be a good investment.
But before this is undoubtedly the primer is 40 C. The annealing temperatures are not always an absolute cutoff. It could even above 40 ° C.. Try it out.
Post a Comment